American Aquarium Products

Aquarium, pond Information, including Columnaris & fungus treatment

FLEXIBACTER- COLUMNARIS & FUNGUS (Saprolegnia):
Treatment, Identification, & Prevention of this Gram Negative Aerobic Bacterium & Fungal Infections

Sections Include: Overview & Complete Article/e-book Download
COLUMNARIS
* Identification of Columnaris (AKA Saddleback Disease & Cotton Wool Disease)
* Treatment of Columnaris
* More about Columnaris including Prevention/Water Quality
FUNGUS/ SAPROLEGNIA
* Saprolegnia (Fungus, mold)
* Treatment
* Lifecycle of the asexual Spores


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RELATED PRODUCTS & INFORMATION

Columnaris  Aquarium Treatments for saleFor Aquarium/Pond treatment products to help with treatment & prevention of Columnaris or Saprolegnia, such as Kanaplex & Furan 2 for Columnaris treatment; or products such as Medicated Wonder Shells or Triple Sulfa for Saprolegnia treatment (among other medications)

For the Identification, Treatment & Prevention of another common Aquarium/Pond Ailment:

Aeromonas, Vibrio, Septicemia, Furunculosis




UV Sterilizer use for Columnaris treatment, prevention
How UVC Sterilization Works

The MOST ACCURATE, RESEARCHED, & IN-DEPTH article on the subject of UV Sterilizer, Clarifier Use in Aquariums or Ponds ANYWHERE!!
Based on over 35+ years professional use too!!
A MUST read for anyone serious about the use of Ultraviolet Sterilization to aid in disease prevention and improving fish disease resistance, ESPECIALLY Columnaris!


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Wonder Shells for Columnaris and Fungus prevention
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TMC UV Sterilizer for Columnaris treatment, preventionTMC PREMIUM UV Sterilizers
Solid durable construction Vecton & Advantage UV Sterilizers, Clarifiers, for aquariums and ponds


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Aquarium Heater; Digital, Quartz, Submersible
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Columnaris, Fungus, Saprolegnia fish display
By Carl Strohmeyer

 
American Aquarium Products:
Frequently Asked Questions

Updated 11/15/14

The fish in the lower right corner of the picture is displaying Saprolegnia while the rest are displaying Flexibacteria-Columnaris


OVERVIEW

Columnaris along with Saprolegnia in particular (Saprolegnia is often confused with Fungus) are two of the most misunderstood and often mistreated aquariums diseases, even Wikipedia has marginal information about these pathogens and their CORRECT treatment and prevention (as of my last reading).
Prevention is the key with both of these unrelated but similar symptom potential fish pathogens.

Please read both sections/articles in full for a better understanding of these common pathogens.

With Columnaris, it is important to learn the effect stress has in outbreaks as well as the importance positive mineral ions and the role these cations play in adhesion of Columnaris by reducing surface potential and repulsive forces.
See the Minerals/Redox Section of Columnaris prevention for a further explanation.

For Saprolegnia in particular, understanding the role of decaying organics, the lifecycle of Saprolegnia zoospores, and even pH play in prevention and treatment of the often very misunderstood Saprolegnia mold/fungi.
As well, after reading both sections, hopefully the reader will understand how the success of treatment of Saprolegnia, as in the disease, Aeromonas is often improved considerably by improving water quality.

Further Reference for Aeromonas:
Treatment, Identification, and Prevention of Aeromonas, Septicemia, Furunculosis, Vibrio

While success in "beating back" Columnaris is more aided (besides treatment) by removing stressors, such as a bullying fish or lack of electrolytes.
Also the progression of Columnaris is often simply aided by weak genetics (a VERY common cause with Bettas) which unfortunately there is little that can be done here other than culling weak fish, which sadly in many instances should be done to stop the spread/progression.

This is also where I find incorrect confusion between Saprolegnia and Columnaris since both look outwardly similar; persons and websites often state that they aided the cure of Columnaris by cleaning a dirty aquarium. This indicates that the fish more likely had Saprolegnia (or even Aeromonas) as you will see from reading the article in full.



WHAT IS COLUMNARIS?

Columnaris, Flexibacteria, characteristic white saddleback lesion Columnaris (often referred to in the past as "cotton wool disease" or "cotton mouth disease") is a warm water, gram negative strictly aerobic and nonhalophilic (meaning they do not live in saltwater conditions) bacterium often appears like a fungus (or more correctly; Saprolegnia) however it is not a fungus, although many treatments for fungus are effective for mild cases of Columnaris (Flexibacteria).
This is why the often recommended Erythromycin is a strange choice of treatment as it is rarely effective for either Fungus of Columnaris.

The picture above left is of fingerling Rainbow Trout displaying a characteristic white saddleback lesion caused by Columnaris bacterium & of a Rift Lake (Malawi) Cichlid displaying classic Columnaris symptoms after stress and injury often caused by an over-crowded cichlid tank

Columnaris under microscope with gram negative stainHowever do not confuse Columnaris with the spores of Saprolegnia the cotton wool ball with hair like growth structures seen on damaged fish. Without a microscope, Columnaris can look like Saprolegnia and is often treated as fungus, sometimes with poor results.
The best way to tell with the naked eye (using a magnifying glass) is the hair like growth structures of Saprolegnia/Fungus.

WITH a microscope, scrapings from a columnaris lesion then placed under a microscope will reveal long, thin, rod shaped motile bacteria. The bacterial clumps form microscopic columns or dome shaped masses, hence the name columnaris.

Columnaris has similarities to Aeromonas bacterial infections in that both are opportunistic (both generally present in some location of an aquarium), however since Aeromonas is a facultative bacterium & often anaerobic, it is more prevalent in a tank with high amounts of pollution, DOC and especially poor circulation.

While Columnaris being only aerobic (preferring high oxygen environments) can occur in tanks with good filtration/circulation, however it is common in a tank with poor Redox/mineralization, overcrowding, high temperatures, and stress (such as an aggressive fish tank where many inhabitants are constantly bullying others).
As well weak genetics from in-bred fish (as with many Guppies and Bettas) or fish such as "feeder" or "carnival" goldfish that are often permanently damaged for early life crowding are also often at risk for Columnaris infections.

Unfortunately the above point is missed by many who often cite dirty, stagnant, or otherwise poor water conditions as cause of Columnaris, but since Columnaris is aerobic, it simply cannot thrive in poor water conditions that are low in oxygen as can Aeromonas or Saprolegnia.
This is NOT to say that sudden shifts in parameters such as spikes in ammonia (assuming an aerobic environment), can will not trigger Columnaris, as this too is a stressor that can allow for an opportunistic Columnaris infection.
HOWEVER, as I have noted many times in my years in aquarium maintenance and "sick fish" service calls; dirty, foul, under filtered, low oxygen environments, RARELY trigger Columnaris infections.

Adding aeration without correcting mineralization problems based on false assumptions about Columnaris while ignoring ESSENTIAL mineral ions (Calcium in particular, but sodium chloride too) will only further force an out of balance Redox and not help with a cure of a true Columnaris infection!!

In lieu of often unavailable scientific microscopic identification, the above point is often noteworthy in identifying aerobic Columnaris from often anaerobic Aeromonas or the Mold Saprolegnia (generally referred to as "Fish Fungus").

Please read further for a better understanding of this bacterial disease as well as treatment and prevention (there is also a section about Fungus/ Saprolegnia as well)


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Identification (& Causes)

Columnaris, Flexibacteria platy, treatment
• A white spot/wound in the body maybe 3/16 inch, that within a week the white area increases to a larger strip in size.

• In gills, Columnaris can cause disintegration of the gill filaments. As the disease progresses the gills can change from their natural color to a light or dark brown. Since it is difficult to absorb enough oxygen from the water using damaged gills, the fish will start breathing rapidly and the fish might also swim up to the surface gasping for air.

Treatment of Columnaris Necrosis of the fins in a goldfish• A white to grey cottony growth (that appears like a fungus) but it appears to be eating away the fish’ skin, as well appears the outside skin is eaten away down to the 'meat'.
(The picture to the left shows necrosis of the fins, please click the picture to enlarge)


Necrosis of fish tissue and cells, Columnaris diagnosis
• Necrosis (premature death of cells and living tissue) of the fins which is accompanied by white, cotton-like accumulations of bacteria and detritus. Fin Rot is a common secondary problem of Columnaris (see the picture in the treatment section)
(See the picture to the left)

• Ulcerations on the skin that slowly or sometimes quickly result in major tissue (epidermis) loss.

• Sometimes the infection where Columnaris is present is a blackish to fleshy in color.


Columnaris, skin lesions will often appear white and gray colored• A lesion that appears like a white/grey “Saddleback” near the dorsal fin (which leads to the other common name for this disease: “Saddleback Disease”.
As well the skin lesions will often appear white/gray colored with an edging of red, which will often change to ulcers caused by the bacteria decaying the underlying tissue.
(The picture to the left shows white/gray fungus "like" skin lesions, please click the picture to enlarge)

• Occasionally Columnaris can appear as a mouth "fungus", however this may actually be Saprolegnia or even Streptococcus

• IMPORTANT; often Columnaris infections are present in well circulated, oxygenated tanks, unlike Aeromonas Infections which are often anaerobic and are much more common in tanks with poor circulation, high bio loads, and large amounts of organic wastes.
Reference: Aeromonas, Vibrio, Septicemia, Furunculosis Bacterial Infections

The bottom line is Columnaris is an aerobic bacterial pathogen that does NOT thrive on organic wastes (this despite claims of inaccurate, poorly researched articles about Columnaris such as one found at about.com!!).
I want to make clear, since many web sites note that Columnaris is generally found in tanks with poor water quality, that this is an incorrect way to diagnose Columnaris (barring a microscopic slide) since this pathogen is aerobic and can become pathogenic in otherwise well maintained tanks (as per many measures of water quality), yet has other stressors that allow for a Columnaris infection such as injury from harassment.

In fact since Columnaris is strictly aerobic, the addition of more aeration/circulation will do nothing to stop Columnaris other than to possibly help the fish fight this disease pathogen.
Unfortunately this key point is so often missed, as this totally incorrect statement from a popular web site shows:
“Columnaris reproduces poorly in the presence of oxygen in the water, so keeping the water's oxygen content up by creating a current can help prevent Columnaris” WRONG!

Columnaris often results in wounds when stress is common in an aquarium or from handling, shipping or any other stress inducing factor for fish.
So a diagnosis of a growing sore as Columnaris in a tank that has fish that are under constant harassment by other fish is often a correct assumption (common in many Lake Malawi Mbuna fish in injuries incurred).

• Sores, wounds, or infections that grow even while under treatment of gram positive medications such as Melafix or Erythromycin may be an indicator of a Columnaris infection as well. Unfortunately Maracyn (which is Erythromycin) is incorrectly prescribed to treat Columnaris by many that do not know that Columnaris is gram negative bacterium while Erythromycin is a gram positive antibiotic.

• Temperature Spikes or sustained high temperatures can often allow for a Columnaris infection to take hold in an otherwise healthy aquarium especially if other stressors are present such as injury, stress, age, etc. (in fact sudden increases in temperature is a common cause of a Columnaris outbreak in an aquarium or pond).
Columnaris thrives in temperatures above 80 F (I have observed 85-90 to be a range where Columnaris is most virulent). A temperature in the 80s is more a factor in cold water fish such as Goldfish.

• Finally, since Columnaris is an opportunistic aerobic infection even a well maintained aquarium can suffer from a Columnaris infection with even a slight opportunity for infection (assuming this bacterial pathogen is present). A common portal for a Columnaris infection is simply an older and weaker fish. Old age allows many opportunistic infections to get a foothold in at least the fish in question.
This is important to note, since treating a fish that has his/her immunity and normal body functions in decline may often be futile. This does not mean it is not worth while attempting a cure, only that an aquarium keeper should not beat him or herself up over failure to cure a favorite fish that has been well cared for since bringing the fish home. I have seen this with Bettas in particular over the years since these popular and personable fish often win us over in our hearts, but have a short lifespan even with the best of care (this lifespan can vary depending upon whether kept in an aquarium or bowl).

Treatment Information:

Use of salt in treatment of Catfish Columnaris, mortality rateSTEP ONE:

The obvious first step is lowering stressors and improving water quality as outlined in the "What is Columnaris", "Identification & Causes", & later "Prevention" Sections. This includes lowering water temperature to 75F (24C).

STEP TWO:

Additional salt is helpful at a dose of 1 tablespoon per 5 gallons OR HIGHER.

In fact a study at the Alabama Agricultural Experimental Station, Auburn University has shown increasing salt concentrations used with Channel catfish (along with heat reduction to 75 F) can treat Columnaris (Flexibacter) infections. This study flies in the face of anecdotal advice about not using salt with catfish.
See the chart to the above/left for the mortality rate of Catfish with Edwardsiella ictaluri (which is a similar gram negative rod bacteria to Columnaris) treated with salt at different levels, please click to enlarge.
References:
*Edwardsiella ictaluri
*Alabama Agricultural Experimental Station, Auburn University

You can see from the diagram that the best results were achieved at a dose of 3000 milligrams per liter; based on the weight of salt this converts .67 teaspoons per liter or 2.54 teaspoons per gallon. This is Much more salt than many aquarists commonly believe a Catfish can tolerate.

Please keep in mind that this amount of salt is NOT meant for long term use, rather just the duration of time it takes to reach a cure for the Columnaris infection.
This amount of salt is most simply used in a 30 minute bath, however as per this study it can be used in the main display aquarium/tank. If added to the main aquarium, I recommend building up to high level suggested by this study over a 2 day period.

Betta with Columnaris, treatment there ofSTEP THREE:

A Fish dip or bath in Mebromin, Potassium Permanganate, or Methylene Blue (not to be confused with malachite green) has also helped speed cure in most instances for my clients fish (or my personal fish) and SHOULD BE part of most Columnaris Treatment regimens!
Caution, do not use Potassium Permanganate with open sores present.

Product References:
*Potassium Permanganate; from AAP
*Kordon Methylene Blue 4 oz, from AAP


In fact with many instances of Columnaris the Methylene Blue Bath (or the even more strong, but more carefully administered Potassium Permanganate bath) was the main factor of treatment that affected a cure as per many tests.

See the picture above/left for a Betta also displaying secondary Fin Rot that literally was on "deaths door" (laying on the bottom with little response) that recovered with a treatment regimen of Kanamycin & Salt as well as regular fish baths which included Methylene Blue, Salt, and Kanamycin.
Please click to enlarge.



The Bottom line is a Fish Bath and maybe a swab too is a MUST part of any moderate to serious Columnaris infection treatment!

With Methylene Blue or Potassium Permanganate I prepare a double strength bath and place the fish in this solution for 30 minutes).
I strongly recommend this bath as a FIRST course of action.
Furan Two AND Kanamycin SHOULD be added to this 20-30 minute bath for more serious or stubborn cases, HOWEVER do not combine these medications or any others with Potassium Permanganate, ONLY Methylene Blue can be combined.

Product Resources:
*Furan 2, Nitrofurazone; from AAP
*Kanamycin,; Kanaplex from AAP


Please see this article for more about Baths:
“Fish Baths, Dips, Direct Treatment Applications”

As well as the above noted baths, direct applications (swabs) of Methylene Blue or Bio-Bandage for mild cases of Columnaris with Hydrogen Peroxide or Diluted Potassium Permanganate to external areas of infection can help with recovery and may be your only chance to check the spread of a more serious infection of Columnaris.

Product Resource: Bio Bandage from AAP

Reference for use of Hydrogen Persoxide: Aquarium Medications 3, Hydrogen Peroxide

STEP FOUR:

Many fish diseases, it should be noted, are caused by different bacterial or fungal pathogens that often exhibit similar symptoms, so identification of a specific bacterial or fungal pathogen is not often possible from mere visual inspection of the symptoms on the fish.

By using broad-spectrum treatments such as a Furan Two & Kanamycin COMBINATION against diseases with similar symptoms affecting fish, precise identification of specific bacterial or fungal pathogens causing the disease that often display similar symptoms may not be absolutely necessary.

Columnaris Treatment, arrowPlease note that the combination of BOTH Kanamycin AND Nitrofurazone MUST be used for effect against true flavobacterium columnare infections, otherwise the minimum inhibitory concentration (MIC) values of these medications individually is too low.

I have also noted reports that while I have enjoyed good success with the above treatment method, assuming water parameters were spot on (including Redox and water temperature), that the failure rate is increasing.
This can be and likely has a lot to do with over breeding of certain fish such as many Bettas, resulting in weak genetics.
However antibiotic resistance is also likely on the increase, which is why this antibiotic combination should not be used on a regular basis every time your fish "burps".

Since the use of Nitrofurazone/Kanamycin is a synergistic combination, improving synergistic combinations via new research into human antibiotics resistance is yielding some interesting results with Oregon Grape Root which aids in antibiotic effectiveness as it contains a specific multi drug resistance pump inhibitor (MDR Inhibitor).
Resistant bacteria work by utilizing a pumping mechanism in its cell that when antibiotics enter that cell the pump immediately pumps out the antibiotics so it can have no effect on the MRSA cell. Oregon Grape Root works by blocking the bacteria's ability to pump out antibiotics.
Currently the best suggestion for use is opening a 400 mg Oregon Grape Root Capsule into 10 gallons of water along with the antibiotics, but keep in mind, aquarium use is still in its infancy!!!

Reference: Oregon Grape Root - It could save the world

Online Source for Oregon Grape Root (not affiliated with AAP):
Solaray Oregon Grape Root Capsules


OTHER TREATMENTS;

Ciprofloxacin is a proven effective synthetic broad spectrum antibiotic against gram-negative bacterium such as Columnaris and can be used in tank or in baths.
The difficulty is finding this antibiotic in small quantities as most is sold in large volumes at high prices (beware of eBay sellers at low prices of this antibiotic, as it is often outdated as per the manufacturer).

Triple Sulfa may also yield positive results for treatment of Columnaris if used early (although this is not as effective of a treatment for Columnaris as it used to be or as the Kanamycin/Nitrofurazone combination).
My professional use of Triple Sulfa has found that it is occasionally a good choice along with baths and some salt for mild to moderate infections and is rarely harsh on aquarium environments including plants.
I stand behind Triple Sulfa as a possible alternative treatment. This despite a rude/condescending comment brought to my attention from Guppies.com. As well, this person is incorrect as per the use a level 1 or higher UV Sterilizer, as it is excellent prevention for Columnaris, including the FACT it improves the Redox Balance.
As to his condescending remarks about me personally, Yes, I have done copious amounts of research (which many are cited here, including the use of Triple sulfa), including my consulting with one of my mentors, Dr. Herzog
.

Product Resource: Triple Sulfa from AAP

Pimafix can aid as a natural "in-tank" treatment for VERY MILD cases of Flexibacteria in combination with lower temps, salt, and baths (I would NOT recommend it for more moderate to serious cases). Do not confuse Melafix with Pimafix, as Melafix is more of an antiseptic with no effect on Columnaris whatsoever!
Pimafix is sometimes effective for a broad range of mild bacterial and fungal infections that typically afflict fish and other aquatic animals (especially gram negative). Fish diseases that may be treated in accordance with this product include bacterial fish diseases, such as fin and tail rot, mouth fungus (often caused by the bacterium Flavobacterium Columnaris); fungal fish diseases (such as those caused by microorganisms of the genera Saprolegnia and Achyle) and the like.
Product Resource: Pimafix from AAP

Unfortunately, there is much unfortunately posted in forums or websites about the use of Tetracycline or Oxytetracyline for the treatment of Columnaris however this is based on old research and misunderstanding of the large Tetracycline class of medications.
Tetracycline is primarily gram positive while Columnaris is a gram negative rod bacteria (although Oxytetracycline is more broad spectrum than Tetracyline Hydrochloride, it is still primarily a gram positive treatment).
This said Minocycline is member of this class of Tetracycline antibiotics and many persons assume all antibiotics in this class are the same, which is not true. Minocycline (sometimes spelled Minocycline) is more gram negative than its cousins and has shown effectiveness for Columnaris.
Maracyn 2 is a product that contains Minocycline.
That said, the Tetracyline family of antibiotics should never be your first choice of medication treatment, despite some misinformed information stating otherwise (please note that I make this statement based on researched medication facts as well as MUCH practical experience dating back to 1979).

References:
*Aquarium Medications Part 2; Tetracycline
*Aquarium Medications Part 2; Oxytetracycline
*Aquarium Medications Part 2; Minocycline

ADDITIONAL FOLLOW UP TREATMENTS;

Among other treatments I have used, especially to prevent reoccurrence once the fish are OK might include “Medicated Wonder Shells”, which contain Acriflavin (as well they contain Methylene Blue).
Acriflavin is active against flex bacteria in mild cases.
While I do NOT recommend Medicated Wonder Shells for a full blown infection of Columnaris these can be an excellent follow up treatment about 7-10 days after completion of regular treatment and baths. These are helpful in part for a Columnaris infection due to the positive mineral ions they deliver along with medication.
I do suggest a water change and adding carbon to filters prior to the introduction of a Medicated Wonder Shell, or other follow up treatment.
Reference: Aquarium Medications Part 3; Acriflavin

Product Resource: Medicated Wonder Shell; ONLY available at AAP

FINALLY, in summary of treatments; one aspect I found over and over, including when I have compared notes with other aquarium maintenance professionals is that attempting to mix a little of one method of treatment with another almost always results in failure.
In other words, if you have found a treatment you have found that works that differs from what I have stated, GREAT!
But do NOT attempt to mix what is suggested here by leaving out some aspects of these treatment steps (or another methods) and expect success. Also as I have already noted, do NOT leave out the importance of correct water parameters, in particular positive mineral ions play in the success of a Columnaris treatment regimen.

PREVENTION & FURTHER COLUMNARIS (Flexibacter) INFORMATION:

Columnaris, Flexibacteria lesions, prevention Flavobacterium columnare gained the nick name “Columnaris” because wet mounts of Flexibacter prepared from diseased fish appear as column-like, "haystack" colonies.

Columnaris, which is a gram negative aerobic bacterium, is often prevalent in systems with poor mineralization.

Reference this article: Aquarium Chemistry; The Importance of Calcium, Electrolytes and more.

As well; crowded conditions (often in re-circulating systems), aggressive inhabitants, poor handling, poor Redox Balance, sudden temperature spikes (or prolonged temperatures much above 80F), and the stress from high bio loads are all conditions that can allow for an opportunistic Columnaris infection.
Please note; high bio loads is more symptomatic of Aeromonas or Saprolegnia infections.

Reference: Aquarium Redox Balance

Columnaris bacterium have caused problems for fish farmers for many years. It is not easy to control, and because the disease is related to stress, an effort to identify and correct the source of the problem is necessary to prevent excessive or chronic mortalities.
Fish are particularly vulnerable to Columnaris following handling and transport, especially since Columnaris is opportunistic warm water pathogen that is often always present in the water, it simply needs an “opportunity” to become pathogenic.

Abrasion from nets, transport, crowding, aggression by tank mates, and adverse water quality conditions (such as lack of necessary electrolytes/minerals) create a situation which is very conducive to Columnaris outbreaks.
With this in mind; lowering stress (such as removing an aggressive inhabitant), improving minerals/electrolytes, and keeping a clean well filtered aquarium are first steps in treating or preventing Columnaris.

Parameters to Consider for Prevention and Treatment of Columnaris:
Please note
that if ANY of these parameters are "off", it may difficult or even impossible to effectively cure a serious Columnaris infection!

*Decrease Fish Load, Crowding Columnaris outbreaks have been shown in University studies to be prevalent in crowded, re-circulating systems, which is a reason I recommend against these types of systems even for fish stores.
This is also common in “African Cichlid” tanks or similar where there is constant stress due to constant battling for hierarchy.

*Ammonia, nitrites; should be 0 ppm

*Nitrates (long term exposure of nitrates over 80 ppm can reduce resistance to disease, under 40 is better)

*Lower your temperature; under 75 F (24 C) (only during treatment).
Goldfish and similar cooler water fish should always be kept under 80F (27C) whenever possible.

*pH (depending upon fish kept) Stability is more important than the actual pH. In other words, do NOT chase you pH, find a KH above 50 ppm and keep your pH stable at whatever pH you get, whether 6.5 or 7.5

* KH: generally a KH of 50 + (what is best here depends upon fish kept). This is related to pH and maintains pH stability via adequate carbonates

*Positive Ionic Composition of the Water & GH: this is a little more complex than this article will deal with, however this is related to both the GH and Redox. In a nutshell MG++ (positive magnesium ions) and CA++ (positive calcium ions) play a part in adhesion of Columnaris by reducing surface potential and repulsive forces.
This is important, please read this article for further information:
Aquarium Chemistry; GH, Calcium, Magnesium, Positive Mineral Ions/Cations.

*Redox Balance; although not generally a major concern for the average aquarist, it is important to understand when problems persist especially since newer research shows its importance in disease prevention. This is an important consideration for an aerobic bacterium such as Columnaris, since often other more obvious water parameters may be good while this one is not.

Redox balance is related to GH (although if all positively charges ions are lost from calcium and other minerals that make up GH, you can still have a higher GH of say 300 ppm and still have a poor Redox Balance).
But the point I want to make is that websites such as Wikipedia and many others are dead wrong to imply that "The bacteria can persist in water for up to 32 days when the hardness is 50 ppm or more" is a causative factor for Columnaris.

While Columnaris Bacterium certainly need these minerals, so do fish and to make the strange leap of thought to state any GH over 50 can lead to Columnaris. This is simply bad science, not to mention proves a lack of practical experience on the part of authors of these articles.
The fact is 50 ppm is a very low GH, even for many soft water fish and more importantly these minerals are essential for correct osmoregulation and a supplier of essential positive electrolytes necessary for fish immunity that Redox research has proven. It is noteworthy that these mineral cations play an important role in adhesion of Columnaris by reducing surface potential and repulsive forces, so for an aquarium keeper to attempt to lower minerals and positive mineral ions is misguided at best.

Please Reference: Aquarium Redox Balance; Importance in Fish Disease Prevention

*Consider ALL steps outlined in this article: “A Healthy Aquarium; Disease Prevention”.

The more steps you follow in this article, ESPECIALLY the use of a TRUE UV Sterilizer, the lower your chance of opportunistic disease outbreaks such as Columnaris (or Saprolegnia/Fungus).
Unfortunately what passes for a UV Sterilizer lately (for under $50) is nothing more than a clarifier and will provide NO aid to the prevention of Columnaris whether directly or as a major aid to a healthy Redox Balance
Please read: UV-C Sterilization; Facts & Information

For the previous stated water parameters, water changes are often very helpful (although watch cross contamination if you have more than one aquarium).

*Good filtration (two filters is always a good option), is very important for treatment and prevention. It is important to catch this disease early, as Flexibacter advances it attacks the internal organs making for more difficult treatment. The Nitrofurazone/Kanamycin combo is about the only antibiotic that will work at this time (unless you have access to Chloramphenicol).
This said, circulation and dissolved oxygen do not play as big a role as with the diseases Aeromonas, Furunculosis, or Vibrio, since Columnaris is a aerobic bacterium that needs oxygen while these others are anaerobic meaning they do best in environments without oxygen.
Reference: Aeromonas, Furunculosis, & Vibrio Infections

Please note that Anabantids (such as Gourami and bettas) seem to be especially susceptible to this disease, which is good reason to practice good cleaning practices with these fish even though they have the ability to get oxygen from the air.
Wonder Shells are great to use in Betta bowls as they add needed electrolytes and calcium and improve water quality between cleanings.

Product Resources:
*Wonder Shells; Unique Version from AAP
*Kanamycin, Kanaplex from AAP


*Genetically Weak Fish, often over breeding or exposure to poor conditions such as with "feeder goldfish" can set up a fish for an opportunistic Columnaris infection. Sadly with genetically weak fish or fish exposed to poor conditions that permanently damage internal organs often NEVER recover from Columnaris, even with the best efforts.
From my experience and speaking with others in my profession, Crown Tail Bettas seem to be especially susceptible to fatal Columnaris infections.

For further reading about Columnaris, please read these outside resources/references:
*http://etd.lsu.edu/docs/available/etd-11112004-113024/unrestricted/Farmer_thesis.pdf
*Columnaris in tilapia; Use of Triple Sulfa for treatment
*http://www.ars.usda.gov/research/projects/projects.htm?ACCN_NO=409119&fy=2006
*http://www.aces.edu/dept/fisheries/aquaculture/pdf/479bfs.pdf
*http://en.engormix.com/MA-aquaculture/articles/f-columnare-myxobolus-tilapiae-infection-t2134/p0.htm
*Prevalence of Edwardsiella ictaluri and Flavobacterium columnaris
*“Influence of water quality and temperature on adhesion of high and low virulence Flavobacterium columnare strains to isolated gill arches”, Decostere, A., Haesebrouck, F., Turnbull, J. F., (1999), Journal of Fish Diseases, 22:1; 1 – 12



Section Two: SAPROLEGNIA (Fungus):

 Saprolegnia , fish fungusSaprolegnia (often classified as a mold; from the Genus “Saprolegnia”) is often confused with Columnaris and for good reasons, they can often be similar in appearance. This similar appearance is why I combined these two unrelated pathogens into this article.
Close inspection though, will reveal that a fish with Saprolegnia will have hair like growth structures similar to what you might find outside growing on a decaying piece of wood in the forest. This is what differentiates Saprolegnia (referred to as fungus) from Columnaris as you will not see the thin hair like structures upon fish with Columnaris.
(Please click picture to enlarge)

Another consideration in differentiating Fungus (Saprolegnia) from Columnaris is that Saprolegnia & fungi often grow on dead tissue, UNLIKE Columnaris which MUST have living tissue to live on.
This is an important distinction, since it is often found on dying or dead tissue of a live fish, as well as the remains of a decomposing fish, shrimp, etc.

One more consideration for the identification of Saprolegnia/fungi is that out breaks often occur in aquarium/pond with large amounts of decaying organic matter, with low pH and poor circulation in the area of decay.


Saprolegnia, fish fungus, treatment & preventionSaprolegnia is not a true fungus (fungus are from the Kingdom “Fungi” such as Oomycetes), while Saprolegnia are from the Kingdom “Protoctista” which is a catch all Kingdom for the multicellular organisms which don't fit into the Animal, Plant, or Fungus Kingdom. This includes molds, green algae, red algae and more.
Please Read Reference #1 at the end of this article, as this is an important definition that so many aquarium website information articles on this subject get wrong.

These organisms, considered to be saprophytic "water molds," are a normal, ubiquitous component of aquatic ecosystems (Saprolegnia are present in ALL aquariums and ponds). Saprophytes live off of decaying organic material.
Saprolegnia, fungus, close up of fish tail, identification
If you have ever seen a piece of fish food left in the aquarium and was covered in what looks like cotton, those are all fungus filaments doing their job of breaking down organics, and although they are a natural part of the aquatic environment, an overabundance of Saprolegnia (Fungus) caused by decaying matter (especially in new aquariums) can result with the Saprolegnia to start trying to break down your living fish and can also appear as cottony growths on the fish as well.
The picture to the left is a double close-up of a fish tail with Saprolegnia/Fungus, with inner picture a multiple magnification of how the Saprolegnia “mold” looks

Saprolegnia will often get started if there is a large amount of decomposition of nitrogenous organic material such as dead fish left in the aquarium or large amounts of high protein fish food such as krill/shrimp. Injured fish with open sores will amplify this potential problem.
This is why I generally recommend against using raw shrimp cycling method recently re-popularized on the internet by a few websites and some forums such as Yahoo Answers. I recommend against the use of raw shrimp for seeding an aquariums nitrogen cycle as this can result (as per my own studies) in Saprolegnia getting to strong a hold in a new aquarium.
Please read further about the lifecycle of Saprolegnia zoospores to also explain the risks, as well as the references.

Further Reference: The Aquarium Nitrogen Cycle; Raw Shrimp Cycling

Treatment

Treatment is somewhat similar to Columnaris, although Saprolegnia responds well to these chemical remedies that are not generally effective for Columnaris (as well as a few antibiotic/organic remedies):

  • Acriflavin (found in Fungus Cure) for mild to moderate Saprolegnia infections

    Acriflavin can be combined with Malachite Green (Victoria Green), or Triple Sulfa for moderate to severe infections. "Fungus Cure" contains both Acriflavin and Victoria Green.

    Product Resource: Fungus Cure from AAP

  • A swab with Methylene Blue or Hikari Bio Bandage in mild cases of Saprolegnia.
    If after a couple days the swab is ineffective, this swab could be stepped up to Hydrogen Peroxide or stepped more to Potassium Permanganate.

    Further Reference for Swabs:
    Fish Baths; Dips, Swabbing (Swabs), etc.

    Product Resource: Bio Bandage from AAP

  • Oxidizers such as Potassium Permanganate or stabilized oxygen chlorine (Maroxy).

    Product Resource: Clear Water Potassium Permanganate

  • I recommend in most moderate to severe Saprolegnia/fungus infections a Medicated Bath using Methylene Blue OR Potassium Permanganate (not mixed). Methylene Blue can be combined with Nitrofurazone (Furan 2) for increased effectiveness.

    Potassium Permanganate can also be used as a swab in mild isolated cases of Saprolegnia/Fungus then as a bath for serious cases.
    This bath can also be helpful even in mild cases to increase the chances of success of many mild to moderate treatments such as Pimafix or Kordon Rid Fungus.
    Please read this article for more about fish baths: Fish Baths, Swabs, Dips

  • Malachite Green based treatments such as Medicated Wonder Shells, ParaGuard or Quick Cure.

    Product Resources:
    *Medicated Wonder Shells; ONLY at AAP
    *SeaChem ParaGuard ParaGuard Fungus, Saprolegnia Treatment
    *Quick Cure, at AAP

  • Organic Remedies such as Pimafix or Kordon Herbal Ich Attack (AKA Rid Fungus) are often successful in mild to moderate infections.

    Product Resources:
    *Pimafix; at AAP
    *Herbal Ich Attack for Fungus too

  • Antimicrobials such as Triple Sulfa also works reasonably well for Saprolegnia (fish fungus).
    Product Resource: Triple Sulfa; from AAP

  • For very difficult cases of Saprolegnia (especially when parasites are also suspected/involved), a strong cocktail of SeaChem ParaGuard (or Quick Cure) and Furan 2 OR Triple Sulfa may prove successful.

    Product Resources:
    *ParaGuard at AAP
    *Furan 2, Nitrofurazone at AAP

Please note that many of the above remedies can be combined for more strength, however others cannot, such as the oxidizers (Potassium Permanganate or stabilized oxygen chlorine).
Medicated Wonder Shells have several ingredients combined (Malachite Green and Acriflavin to name two) but is not as strong for serious fungus/Saprolegnia outbreaks (since these are not combined at full strength in Med. Wonder Shells) as combining other full treatments such as ParaGuard and Triple Sulfa or Acriflavin.


Salt, dips, and baths are also effective (in serious cases essential) as part of a Saprolegnia treatment.
I would use plain salt (non iodized) or even; "SeaChem Cichlid Salt" which is a balanced salt with other essential elements necessary for most freshwater fish (not just cichlids as the name implies).
As noted earlier, a Methylene Blue or Potassium Permanganate bath used at double tank strength for 30 minutes twice per day with the bath water disposed of afterwards is also very useful for Saprolegnia (Fungus) treatment (generally Potassium Permanganate is more effective for Saprolegnia).
Product Resource: Cichlid Salt; A Balanced Mineral Salt from AAP

However treatments often useful for Columnaris such as Kanamycin are less effective for Saprolegnia/Fungus.
This said, I disagree with some statements I have read about the use of antibiotics for Saprolegnia stating that these should not be used over concern for the nitrifying bacteria; these statements are based on gram positive bacteria such as Erythromycin which can and will harm your nitrifying bacteria, however the antibiotics which are effective for Saprolegnia and Columnaris are gram negative and would take over use to destroy you bio filter bed.

As for Erythromycin, I have read many fish keeping articles (especially on Betta forums) where Erythromycin (aka Maracyn) is recommended for Fungus (Saprolegnia); This is simply WRONG! While Fungus is not a bacteria and so cannot be labeled as either gram positive or gram negative; the fact is gram positive medications do not treat true fungal/saprolegnia infections and even if this is a misdiagnosis with Columnaris, this is still incorrect since Columnaris is gram negative. Sadly this misinformation is cut and paste around the Internet resulting in many needless fish deaths based on my experience and emails!!

Saprolegnia (fungus) can be even more opportunistic in poor water with stressed or injured fish than many bacterial infections such as Columnaris, so healthy water conditions are even more important for cure (& prevention) and if water conditions are poor, all the medications in world may not affect a cure!

The obvious 0 ammonia/nitrites are important but so is the often forgotten GH and a stable pH (provided by a proper KH for the fish kept). Without adequate calcium (found in GH) your cure will be difficult to achieve. Medicated or regular Wonder Shells may help in this area.
Reference: Aquarium Chemistry; GH, KH, pH, Calcium, Electrolytes for a healthy aquarium

Also important is a clean tank without organic mulm buildup, so make sure there is NO decomposing organic debris which can by itself bring on a Saprolegnia infection, in fact I have documented this which is why I generally recommend against using raw shrimp to start your nitrogen cycle.
Please see this well researched article for more about the Aquarium Nitrogen Cycle:
“The Aquarium (& pond) Nitrogen Cycle”.

This article about aquarium cleaning may also be useful:
“Aquarium Cleaning”

Prevention/Lifecycle of Saprolegnia

Understanding the lifecycle and Prevention of Saprolegnia is probably the best “cure”.

The lifecycle is best described this was: the asexual spore of Saprolegnia release motile, primary zoospores.
These primary zoospores are active only for a few minutes before they encyst, germinate, and release a secondary zoospore.
Secondary zoospores are more motile for a longer period of time than primary zoospores and as well are considered the main dispersion phase of Saprolegnia.
The repeated cycles of encystment and release, called "polyplanetism", allows secondary zoospores to make several attempts to locate a suitable substrate or host.

It is these Secondary zoospores which are considered the infectious spore of Saprolegnia and can reside in grave/substrate, filters, and the water column for some time until this host is found (these spores do NOT require a food source during this time as well). Repeated vacuumings and large water changes can reduce this number but not totally alleviate it unless the tank is sterilized with bleach (especially since these spores can reside anywhere in an aquarium), so denying the zoospores a host via healthy, non stressed disease resistance fish is the next step.
This is major reason why the long ago discredited "Raw Shrimp" aquarium cycling method should NOT be used!
Please click on the diagram for a better view/larger diagram of the Saprolegnia lifecycle. From: http://hmsc.oregonstate.edu/classes/MB492/saprokent/saprolegnia.htm
Further Reference: Aquarium Nitrogen Cycle; Raw Shrimp Cycling

Make sure to vacuum out ALL decaying organic matter, especially high protein organics such as uneaten shrimp or similar. I recommend treating all wounds in fish immediately when discovered, slowly increasing pH (for fish that can tolerate a higher pH, which even Discus can thrive/breed in a pH above 7.0) and finally maintain proper electrolytes(such as calcium cations) in your aquarium as well as the possible addition of salt when wounds are discovered for healthy osmoregulation.
Reference: Aquarium Chemistry; Electrolytes

It is noteworthy that Saprolegnia thrive in lower pH water, especially under 6.0, although any water under 7.2 provides a habitat that can support strong Saprolegnia growth. With this in mind, keeping slightly more alkaline water is both good treatment and prevention for Saprolegnia.

As to correct levels of electrolytes such as calcium (which is all too often missed by many anecdotal aquarium articles), is that CORRECT mineralization induces a healthy mucous layer on the fish epidermis which is so VERY important in fish resistance to Saprolegnia infections, in fact this quote from Oregon Sate University says it best:
“Willoughby (1989) determined that fish have 3 types of defenses against Saprolegnia.
  • First, the physical removal of attached spores by the renewal of mucous.
  • Second, a morphogen in the mucous inhibited the growth of mycelium but not killing it.
  • And third, a cellular response in the mucous is directed at growing mycelium.

Therefore, the mucous acts as a primary physical barrier (Bruno and Wood, 1999; Pickering, 1994), by continuous replenishment of the mucous layer (Pickering and Willoughby, 1982), although not for complete, i.e., 100%, removal of fungal spores (Murphy, 1981; Willoughby and Pickering, 1977).
However, a fish having an intact epidermis is probably the best defense against saprolegniasis (Hatai and Hoshiai, 1994; Pickering, 1994).”


Please follow this link for more from the article from which this quote came:
“Saprolegnia; There’s a Fungus among us” .

This why I cannot emphasize more the need for correct mineralization (products such as Wonder Shells are excellent for this), the use of salt when needed, possibly a higher pH or KH and over all good water quality that includes a healthy Redox Balance.
See also Aquarium/Pond Chemistry

As with Columnaris, the use of a correctly set up, well designed UV Sterilizer (such as the Premium TMC Vecton or Advantage UV Sterilizer) can help with Saprolegnia (Fungus) prevention.
Reference: UV Sterilization; Facts & Information

Product Resource: TMC Vecton or Advantage High Dwell Time UV Sterilizers

For further reading about Saprolegnia, please read these outside resources:
(1) " Kingdom Protoctista is defined by exclusion: its members are neither animals (which develop from a blastula), plants (which develop from an embryo), fungi (which lack undulipodia and develop from spores), nor prokaryotes. They comprise the eukaryotic microorganisms and their immediate descendants: all nucleated algae (including the sea-weeds), undulipodiated (flagellated) water molds, the slime molds and slime nets, and the protozoa. Protoctist cells have nuclei and other characteristically eukaryotic properties; most have aerobiosis and respiration in mitochondria and 9+2 undulipodia at some stage of the life cycle."
From: http://www.bio.umass.edu/biology/conn.river/protoc.html
(2) “What is Saprolegnia? By Kent Mayer OSU Department of Fisheries and Wildlife” ,
(3) “Fungal Diseases of Fish”
(4) Neish, G.A., and Hughes, G.C. 1980. Diseases of fishes, Book 6, Fungal Diseases of Fishes. T.W.F. Publications, Neptune, New Jersey. 159 p.
(5) “The Occurrence of Watermolds with respect of pH of Water ”


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